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Another stain that can be used, and is demonstrated below, is toluidine blue. Have microscope slides and nigrosin-eosin stain prewarmed to body temperature. Anatomic site of the defect: It is always the case that some sperm from an ejaculate are morphologically abnormal, but when that fraction becomes excessive, fertility may decrease.


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Bull and human sperm, for example, have paddle-shaped heads, rodent sperm have hook-shaped heads, and the heads of chicken sperm are spindle-shaped and almost difficult to distinguish from the midpiece. Pipet a drop of stain onto the end of a slide, then pipet a small droplet of semen next to the stain. An nigrosin-eosin stain is commonly used because it is effective, simple and, in addition to allowing sperm to be readily visualized, it is a so-called "live-dead" stain, allowing one to assess membrane integrity at the same time as morphology.

Examine using a bright field microscope typically using a X objective lens. In the most fundamental case, the size and shape of the head, midpiece and tail are examined.

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The problem can involve the head, midpiece or tail. An nigrosin-eosin stain is commonly used because it is effective, simple and, in addition to allowing sperm to be readily visualized, it is a so-called "live-dead" stain, allowing one to assess membrane integrity at the same Busty miriam videos as Big booty butt anal. Finally, a technique preferred by many for evaluating sperm morphology is to use no stain at all, but Hot lesbian sex redtube visualize sperm under differential Slide of human sperm contrast microscopy.

Some authors question the utility Bookgirlssex physiologic basis of this pattern of classification.

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Description:Two types of classification schemes are commonly used: Some abnormal sperm may have defects in more than one site. Primary defects are the more severe and are thought to originate while the sperm was still within the semeniferous epithelium of the testis. An nigrosin-eosin stain is commonly used because it is effective, simple and, in addition to allowing sperm to be readily visualized, it is a so-called "live-dead" stain, allowing one to assess membrane integrity at the same time as morphology. Examine using a bright field microscope typically using a X objective lens.

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